Effect of Glacial Acetic Acid Treatment of Liquid-based Cytology Collections

28 29 Inadequate cervical cytological analysis can be facilitated by glacial acetic acid (GAA)-treatment 30 of primary liquid-based collections to remove mucus, erythrocytes, inflammatory cells, and 31 cellular debris. In the context of a commercial HPV hybridization assay performed on 465 32 tandem specimens with and without GAA treatment, we show that GAA treatment significantly 33 reduces genomic DNA content (P < 0.0001) and creates an increased potential for indeterminate 34 and false-negative results. In the context of cytological workflow, laboratories should consider 35 providing a specimen aliquot for HPV DNA detection prior to GAA treatment. 36 37 on O cber 7, 2017 by gest ht://jcm .sm .rg/ D ow nladed fom